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hiv 2 virus replication  (ZeptoMetrix corporation)


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    Structured Review

    ZeptoMetrix corporation hiv 2 virus replication
    Hiv 2 Virus Replication, supplied by ZeptoMetrix corporation, used in various techniques. Bioz Stars score: 94/100, based on 96 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/hiv+2+virus+replication/HIV+Type+2/pm40507836-170-10-27
    Average 94 stars, based on 96 article reviews
    hiv 2 virus replication - by Bioz Stars, 2026-09
    94/100 stars

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    Virus:

    Article Title: Macrophage-Derived Factors with the Potential to Contribute to the Pathogenicity of HIV-1 and HIV-2: Roles of M-CSF and CXCL7
    Article Snippet: Subsequently, these cells were infected with HIV-2 B2-5, and B7-9, as well as HIV-2 Rod (5 ng p26 per well); incubated at 37 °C/5% CO 2 for 2 h; washed thoroughly; and cultured in MEM medium supplemented with 10% FBS (Hyclone/Thermo Scientific; Logan, UT, USA); 10 μg/mL puromycin (Sigma; St. Louis, MO, USA); 100 μg/mL hygromycin (Invitrogen; Carlsbad, CA, USA); 500 μg/mL G418; penicillin/streptomycin; and L-glutamine (Quality Biological, Inc.). .. Culture supernatants were harvested on days 4, 8, and 12; HIV-2 virus replication was determined by measuring p26 antigen levels using the SIV p27 antigen ELISA kit (Zeptometrix Corporation; Buffalo, NY, USA) as described previously [ ] after we confirmed that this ELISA kit cross-reacts with HIV-2 p26 antigen. ..

    Article Title: Macrophage-Derived Factors with the Potential to Contribute to the Pathogenicity of HIV-1 and HIV-2: Roles of M-CSF and CXCL7.
    Article Snippet: Subsequently, these cells were infected with HIV-2 B2-5, and B7-9, as well as HIV-2 Rod (5 ng p26 per well); incubated at 37 ◦C/5% CO2 for 2 h; washed thoroughly; and cultured in MEM medium supplemented with 10% FBS (Hyclone/Thermo Scientific; Logan, UT, USA); 10 μg/mL puromycin (Sigma; St. Louis, MO, USA); 100 μg/mL hygromycin (Invitrogen; Carlsbad, CA, USA); 500 μg/mL G418; penicillin/streptomycin; and L-glutamine (Quality Biological, Inc.). .. Culture supernatants were harvested on days 4, 8, and 12; HIV-2 virus replication was determined by measuring p26 antigen levels using the SIV p27 antigen ELISA kit (Zeptometrix Corporation; Buffalo, NY, USA) as described previously [93] after we confirmed that this ELISA kit cross-reacts with HIV-2 p26 antigen. ..

    Enzyme-linked Immunosorbent Assay:

    Article Title: Macrophage-Derived Factors with the Potential to Contribute to the Pathogenicity of HIV-1 and HIV-2: Roles of M-CSF and CXCL7
    Article Snippet: Subsequently, these cells were infected with HIV-2 B2-5, and B7-9, as well as HIV-2 Rod (5 ng p26 per well); incubated at 37 °C/5% CO 2 for 2 h; washed thoroughly; and cultured in MEM medium supplemented with 10% FBS (Hyclone/Thermo Scientific; Logan, UT, USA); 10 μg/mL puromycin (Sigma; St. Louis, MO, USA); 100 μg/mL hygromycin (Invitrogen; Carlsbad, CA, USA); 500 μg/mL G418; penicillin/streptomycin; and L-glutamine (Quality Biological, Inc.). .. Culture supernatants were harvested on days 4, 8, and 12; HIV-2 virus replication was determined by measuring p26 antigen levels using the SIV p27 antigen ELISA kit (Zeptometrix Corporation; Buffalo, NY, USA) as described previously [ ] after we confirmed that this ELISA kit cross-reacts with HIV-2 p26 antigen. ..

    Article Title: Macrophage-Derived Factors with the Potential to Contribute to the Pathogenicity of HIV-1 and HIV-2: Roles of M-CSF and CXCL7.
    Article Snippet: Subsequently, these cells were infected with HIV-2 B2-5, and B7-9, as well as HIV-2 Rod (5 ng p26 per well); incubated at 37 ◦C/5% CO2 for 2 h; washed thoroughly; and cultured in MEM medium supplemented with 10% FBS (Hyclone/Thermo Scientific; Logan, UT, USA); 10 μg/mL puromycin (Sigma; St. Louis, MO, USA); 100 μg/mL hygromycin (Invitrogen; Carlsbad, CA, USA); 500 μg/mL G418; penicillin/streptomycin; and L-glutamine (Quality Biological, Inc.). .. Culture supernatants were harvested on days 4, 8, and 12; HIV-2 virus replication was determined by measuring p26 antigen levels using the SIV p27 antigen ELISA kit (Zeptometrix Corporation; Buffalo, NY, USA) as described previously [93] after we confirmed that this ELISA kit cross-reacts with HIV-2 p26 antigen. ..



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